![(A) Confocal microscopy illustrating FLAG staining along with CD31+ and DAPI staining in lung cryosections obtained from mice receiving either a scrambled control (Ctrl), FLAG-Irf1, or FLAG-Irf1/shLif construct. Scale bar = 50 and 20 μm (enlarged panel). Co-localization coefficient for FLAG-Irf1 staining in CD31+ MLECs. (B) FLAG-Irf1 and Lif protein levels in MLECs derived from mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct. (C) Lung vessel permeability pre- and post-LPS in mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct. (D) Flow cytometric analysis of CD31+CD45− endothelial cells pre- and post-LPS in mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct. (E) BrdU+ MLEC quantification in lung cryosections obtained from mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct (n = 6 cryosections per mouse). (F) Survival curves following LPS challenge in mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct (n = 30 mice per group). All experiments except (F): n = 6 mice or six independent biological replicates per group. Data represented as means ± SDs. *P<0.05, **P<0.01 [(A–E) two-way ANOVA with Bonferroni post-hoc test; (F) Log-rank test].](https://port.silverchair-cdn.com/port/content_public/journal/clinsci/137/5/10.1042_cs20220876/1/cs-2022-0876i004.png?Expires=1717067666&Signature=QkgVl6dPP0x-VADlaTGdJiMD4Y7gvqwwj17hOLgz~DgcKXWEsgWRjxgGOJJ4I92lg2TH094skU8lp~jLkftS19uawbxT4uArdLVD2AsfSfwdeJTZe37gpJAMOD~YmQru0UQfVw6E3AjHbsDD9EWliIyl8NG92MTKnhKXyJydG20r2iPz7yoRRQeMZ3oM-IKUCF~xv27sJguCWZV1qNN9biIyqQ9LtqXrRKI4EmiD6sqshZpuupSxWklEqZL81fxBlFtvg4wbaRNI~p39mSdJ-DTNp4spqx4aKTjGUx1DtkWdslKxls2sxSGImpAa80lOutHy0FcwEUKR07e25fyCsQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) Confocal microscopy illustrating FLAG staining along with CD31+ and DAPI staining in lung cryosections obtained from mice receiving either a scrambled control (Ctrl), FLAG-Irf1, or FLAG-Irf1/shLif construct. Scale bar = 50 and 20 μm (enlarged panel). Co-localization coefficient for FLAG-Irf1 staining in CD31+ MLECs. (B) FLAG-Irf1 and Lif protein levels in MLECs derived from mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct. (C) Lung vessel permeability pre- and post-LPS in mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct. (D) Flow cytometric analysis of CD31+CD45− endothelial cells pre- and post-LPS in mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct. (E) BrdU+ MLEC quantification in lung cryosections obtained from mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct (n = 6 cryosections per mouse). (F) Survival curves following LPS challenge in mice receiving either a Ctrl, FLAG-Irf1, or FLAG-Irf1/shLif construct (n = 30 mice per group). All experiments except (F): n = 6 mice or six independent biological replicates per group. Data represented as means ± SDs. *P<0.05, **P<0.01 [(A–E) two-way ANOVA with Bonferroni post-hoc test; (F) Log-rank test].