Structure and function of eu- and prokaryotic gasdermins
Structure of (A) the monomeric human GSDM-D (pdb 6N90), (B) a bacterial GSDM (of Bradyrhizobium tropiciagri, pdb 7N50), and (C) the AlphaFold predicted structure of a cyanobacterial GSDM (of Nostoc sp. NIES-2111) [106,107]. The proteins have an extended β-sheet structure, which reorganizes after cleavage of an inhibitory peptide to form an elongated monomer (E) that forms large, homo-oligomeric rings (D,F). The oligomeric ring partially integrates into and forms a pore within the membrane. The structure of the human GSDM-D ring (pdb 6VFE) is shown in a side view (D) as well as when embedded within a membrane model (F).