RadA ATPase mutants display altered responses to different cofactors
(A) Representative autoradiographs of D-loop time courses. Divalent cations tested are indicated, as is the presence/absence of the ATP regeneration system. Invasion products for each condition were measured at 1-, 30-, 60-, and 90-min time points. (B) D-loop formation quantitation for the K120R mutant protein. Mn2+ data are shown in a separate panel from Mg2+ and Ca2+ to highlight variations. (C) D-loop formation quantitation for the K120A mutant protein. For all data points shown in panels (B and C), a minimum of three replicates were used. Results are expressed as fold increase relative to WT SsoRadA with Ca2+ and ATP regeneration at the 1-minute time point. For panels (B and C) bars with crosshatch patterning are reactions that include the ATP regeneration system. Error bars represent standard deviation in all cases.