Sequence alignment of FtsZ and eukaryotic tubulin
Clustal Omega tool was used to align the amino acid sequences of human α-tubulin isoform 1A (α-tubulin, 451 residues), M. jannaschii FtsZ (MjFtsZ, 364 residues), E. coli FtsZ (EcFtsZ, 383 residues), P. aeruginosa FtsZ (PaFtsZ, 394 residues), M. tuberculosis FtsZ (MtbFtsZ, 379 residues), S. pneumoniae FtsZ (SpnFtsZ, 419 residues), B. subtilis FtsZ (BsFtsZ, 382 residues), and S. aureus FtsZ (SaFtsZ, 390 residues). The residues that show identity are marked in blue using Jalview. N-terminal and C-terminal domains separated by the T7 loop region is indicated in blue, red, and yellow lines, respectively, above the sequence alignment. The G-box GTP binding motif and the C-terminal tails are highlighted.