Analysis of polyadenylation factors expression in E. histolytica and other protozoan parasites
(A) Heat map showing differential expression of polyadenylation factors growing in distinct conditions. Data were obtained from published RNA-seq experiments. (B–D) Real-time qRT-PCR for five E. histolytica genes selected from the heat map and PPI network. The EhRNAPII gene mRNA expression was used as normalization control for all qRT-PCR assays. Data corresponding to stress (serum starvation and heat shock) and virulent conditions were expressed as mean ± SD (n=3) and compared with the control condition using the paired Student’s t-test; *P<0.05, **P<0.01, and ***P<0.001.