(A) Co-precipitation of HSP90, CDC37 and FKBP5 with NT- and CT-strep tagged PSKH2. (B) Co-precipitation of HSP90 proteins with full-length and truncated PSKH2 constructs. (C) Co-precipitation of HSP90 proteins with WT and canonical kinase activity mutants of PSKH2. (D) Reduction in PSKH2 expression in HEK-293T cells with increasing concentration of geldanamycin and (E) celasterol. Densitometry data shown in the right panel of (D) and (E) is mean and SD of PSKH2 band intensity from four independent experiments, first normalised to tubulin and then PSKH2 bands corresponding to control (DMSO) conditions.