Figure 2.
![PC12 cells proliferate in response to EGF, which causes transient RAS and ERK pathway activation, whereas NGF causes sustained RAS and ERK activation and neuronal differentiation (visible as the extension of long neurites). Feedback phosphorylation of the NF1 GAP by ERK occurs selectively in response to NGF stimulation [9]. It interferes with NF1 binding to RAS thereby allowing RAS activation to persist.](https://port.silverchair-cdn.com/port/content_public/journal/biochemj/480/1/10.1042_bcj20220234/1/bcj-2022-0234c.02.png?Expires=1717029558&Signature=3SFveT-bLXpQy0jH9OeySUODhUJquz8DIt4ra7NKDIK3P6m0iFFXgfOj1UhOwxdL-XMyvguyJoqdZHOx9xC0QwCFoX7FdGYd0lBVTSW6BDrQki9aclGbNz2Ka5HmI5uRfnedOxqU-165Rdw25iZZk3FcemSAOoxgeeblxE8wm5DItVFMDahx6arlXoqyfCshuTr5p0aBT5Wfs4Kr3T9u6AOxAat9MuLc~QNCCTCacB0~izzJpZ5qbGGLE8oU5clMa2yEF2outf3Ts0FRMrzFcAwjwPot6D6a56qrSAHQWb4xc-rRvrzI50BoE3f0teknubSY1d25OK4D9srZbxBzXA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
RAS activation kinetics determine cell fate decisions in PC12 cells.
PC12 cells proliferate in response to EGF, which causes transient RAS and ERK pathway activation, whereas NGF causes sustained RAS and ERK activation and neuronal differentiation (visible as the extension of long neurites). Feedback phosphorylation of the NF1 GAP by ERK occurs selectively in response to NGF stimulation [9]. It interferes with NF1 binding to RAS thereby allowing RAS activation to persist.