Structure of the peroxisomal ubiquitin ligase complex.
(A) Cartoon model of the structure of the ubiquitin ligase complex from T. thermophilus, determined by cryo-EM. Shown is a side view, with the three components PEX2, PEX10, and PEX12 in different colors. The ligase complex has two distinct domains: a membrane-embedded domain (TMD) and a cytosolic domain (CytD) formed by the three RING fingers (RFs). CytD is also referred to as the RING finger tower. (B) View of the ligase complex from the lumen. Shown are cartoon models of the components together with semi-transparent space-filling models. Note the open pore. The broken line indicates a short luminal loop that is invisible in the density map, but would partially obstruct the pore.