(A) Chromaffin granules permeate H⁺ in expense of ATP hydrolysis, but not other abundant cations, and they need a Cl⁻ conductance for proper acidification by nullifying positive charges from proton translocation. The CHGB channel is well positioned and good fit to be a critical part, if not the sole one, of this long-missing anion shunt pathway [2]. Adapted from ref. [3] with permissions under the PubMed open access policies. (B) Purified recombinant murine CHGB (mCHGB) reconstitutes anion channels in planar lipid bilayers. The presence of Ca²⁺ or Mg²⁺ leads to inactivation at high polarization potentials. (C) Single channel currents of mCHGB as a function of transmembrane potential (Vm), giving rise to an average single channel conductance of 140 pS (42/166 mM KCl). Panels B and C were from ref. [4] with permission under the PubMed open access policies.