Comparison of the structures of tau fragments formed in vitro
CryoEM structures for tau297-391, dGAE and 266/297-391 [54] assembled under a wide variety of conditions. Listed conditions refer to those entered in table 1 of Lövestam et al. [54] and identified by conditions in brackets. PDB files are provided for each structure and all conditions are 10 mM PB, 10 mM DTT, pH 7.4, 200 rpm and differences are noted in square brackets. (A) 7QJV (2c) 297-391 10 mM PB, 10 mM DTT, pH 7.4, 200 rpm; (B) 7QJW (8a) 266/297-391 [200 mM NaCl]; (C) 7R5H (10b) 266/297-391 [200 mM KCl]; (D) 7QJY (9a) 266/297-391 [200 mM LiCl]; (E)7QJZ (9b) 266/297-391 [200 mM LiCl]; (F) 7QKF (12a) 266/297-391 [200 μM CuCl2]; (G) 7QK5 (10a) 266/297-391 [200 mM KCl, 200 rpm; (H)7QKU (14a) 266/297-391 [20 mM MgCl2 100 mM NaCl]; (I) 7QKJ (14b) 266/297-391 [20 mM MgCl2 100 mM NaCl]; (J) 7QKL (11a) 266/297-391 [100 μM ZnCl2]; (K) 7QL3 (8b) 266/297-391 [200 mM NaCl]; (L) 7R4T (16a) 266/297-391 [10 mM NaHCO3 100 mM NaCl]; (M) 7QKV (15a) 266/297-391 [10 mM MgSO4 100 mM NaCl]; (N) 7QKX (15b) 266/297-391 [10 mM MgSO4 100 mM NaCl]; (O) 7R5H (10b) 266/297-391 [200 mM KCl].