(A) DG75 cells were transiently transfected with 2 μg of the control vector pGL3 Basic or pGL3_CD79Bp reporter constructs in the presence or absence of an EBNA2 expressing construct (pSG52A) and/or an EBF-1 expressing construct (pCMV-SPORT62-EBF-1). Cells were co-transfected with a Renilla control plasmid pRL-CMV (0.5 μg). Firefly luciferase signals were normalised to Renilla luciferase signals. Results show the mean ± standard deviation of two independent experiments and are expressed relative to signal in the absence of EBNA2 or EBF-1. Lower panels show western blot analysis of EBNA2 and EBF-1 expression in transfected cells. Actin was used as a loading control. T-tests were performed and significance indicated as follows; * (P-value > 0.05), ** (P-value ≤ 0.05), *** (P-value ≤ 0.01). (B) DG75 RBP-J KO cells were transfected as in (A). (C) A direct comparison between promoter activity in DG75 and DG75 KO cells (data from (A) and (B)).