MDM2 knockdown reversed the effect of CEACAM5 silencing on the EMT
sh-MDM2-NC or sh-MDM2 was transfected into HNSCC cells after stable lentivirus-mediated transduction. (A) The mRNA levels of EMT-related transcription factors and biomarkers were measured by qRT-PCR. (B) The protein expression levels of MDM2 and EMT biomarkers were determined by WB. The relevant statistics are shown in the bottom panel. (C) Flow cytometry was used to detect the cell-cycle distribution of different cell lines. (D) The wound healing capacity of HNSCC cells transfected with sh-MDM2-NC or sh-MDM2 is shown. (E,F) Diagram showing the mechanism of the hypothesis postulated in the present study (generated by Figdraw). *P<0.05, **P<0.01, ***P<0.001; Abbreviation: NS, not significant.