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Figure 4.

The reaction mixtures contained 100 µg/ml RG-II monomer (≍20 µM), 1.2 mM boric acid, 0–1000 µg/ml histidine (0–6450 µM) (a) or His6.Cl− (His6; 0–966 µM) (b), and 50 mM acetate (Na+) buffer pH 4.8. Controls lacked boric acid or RG-II, as indicated above the gels. Other controls contained 50 µg/ml polyhistidine.Cl− (PH) instead of His or His6. After 16 h at 20°C, 0.8 µg of the RG-II was analysed by PAGE followed by silver staining. The bands are quantified in Figure 3.

Concentration dependence of histidine and hexahistidine as potential chaperones.

The reaction mixtures contained 100 µg/ml RG-II monomer (≍20 µM), 1.2 mM boric acid, 0–1000 µg/ml histidine (0–6450 µM) (a) or His₆.Cl⁻ (His₆; 0–966 µM) (b), and 50 mM acetate (Na⁺) buffer pH 4.8. Controls lacked boric acid or RG-II, as indicated above the gels. Other controls contained 50 µg/ml polyhistidine.Cl⁻ (PH) instead of His or His₆. After 16 h at 20°C, 0.8 µg of the RG-II was analysed by PAGE followed by silver staining. The bands are quantified in Figure 3.

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