(A) FBP1 was differentially enriched in epigenetic regulation of gene expression according to GSEA. (B) FBP1 was differentially enriched in PRC2 methylation of histone and DNA according to GSEA. (C) The relationship between FBP1 and EZH2 was determined based on TCGA dataset. (D) Heatmap of co-expression of FBP1 and EZH2 based on TCGA dataset. (E,F) The physical interaction between FBP1 and EZH2 in OC SKOV-3 cellswasdetected by Co-IP assay using FBP1 or EZH2 antibody, respectively. (G) Protein expression of FBP1, EZH2 and H3K27me3 in FBP1-C and FBP1-KD OC SKOV-3 cells as detected by Western blot analysis. (H) mRNA expression levels of FBP1 and EZH2 in ovarian cells as detected by qRT-PCR. (I) Relative protein expression levels of FBP1, EZH2 and H3K27me3 in FBP1-C and FBP1-KD OC SKOV-3 cells. (J) Expression of EZH2 and H3K27me3 in normal, benign and malignant ovarian tissues as detected by IHC. (K) Expression of EZH2 and H3K27me3 in xenograft tumor tissues as detected by IHC. (L) mRNA expression of FBP1 and EZH2 in xenograft tumor tissues as detected by qRT-PCR. (M) Protein expression of FBP1, EZH2 and H3K27me3 in xenograft tumor tissues as detected by Western blot analysis. (N) Relative protein expression of FBP1, EZH2 and H3K27me3 in xenograft tumor tissues. Note: The blots were cropped from the same gel; magnification: ×400; *P<0.05. ***P<0.001; bar: 50 μm.