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Figure 5.

The recordings are made at ±50 mV in the presence of 50 µM free Ca2+. The planar lipid bilayer was formed from 50 : 50 POPS and POPE. The BKα channel/SMALP complex has inserted into the bilayer backwards and C and O represent the closed and open state. (A) BK single channel currents at −50 mV. (B) The Patlak amplitude histogram of the potassium current flowing through the channel. The histogram has been fitted to two Gaussian curves. (C) BK single channel currents at +50 mV. (D) The Patlak amplitude histogram of the potassium current flowing through the channel at +50 mV.

BK channels (hslo α + β1) purified using SMALP's can form functional BK channels in a planar lipid bilayer.

The recordings are made at ±50 mV in the presence of 50 µM free Ca²⁺. The planar lipid bilayer was formed from 50 : 50 POPS and POPE. The BKα channel/SMALP complex has inserted into the bilayer backwards and C and O represent the closed and open state. (A) BK single channel currents at −50 mV. (B) The Patlak amplitude histogram of the potassium current flowing through the channel. The histogram has been fitted to two Gaussian curves. (C) BK single channel currents at +50 mV. (D) The Patlak amplitude histogram of the potassium current flowing through the channel at +50 mV.

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