(A) Schematic representation shows the differentiation protocol to derive beating cardiomyocytes from cloned CSCs in vitro. (B) Representative confocal image of mouse cloned CSCs-derived cardiospheres (CS) before myogenic differentiation induction (left), showing no cTnI or MF20 expressing cells and of functional cardiomyocytes derived from CS differentiation, showing a homogenous expression of cTnI (red) and MF20 (green). (C) Confocal microscopy examples of CSC-derived iCMs, neoCMs and adult CMs labeled with cTnI (red). (D) Heatmap showing qPCR analysis of main contractile genes in cardiosphere-derived CSCs (Actc1, Tnnt2, Myh7, Actc1, Myh6, Acta2, Cnn1) and cardiac transcription factors (Mef2c, Gata4, Nkx2.5, Hand 2 and Brachyury T) after myogenic differentiation. *Adapted from reference [78].