Cryo-EM structure of the Synechocystis cytb6f–PetP complex.
(A) View of the colour-coded (according to the key in panel F) cytb6f–PetP density map showing cytochrome b6 (cytb6 green), cytochrome f (cytf pink), the Rieske ISP (yellow), subunit IV (subIV cyan), PetG (grey), PetM (salmon), PetN (pale orange), PetL (pale purple) and PetP (dark purple). Detergent and other disordered molecules are shown in semi-transparent light grey. The grey stripe indicates the approximate position of the thylakoid membrane bilayer. (B) View of the cytb6f–PetP density map following local sharpening coloured as in (A). (C) Modelled subunits of cytb6f shown in a cartoon representation and coloured as in (A). (D) Modelled cofactors of cytb6f showing hemes bn and bp (both red), hemes cn and f (both dark blue), the 2Fe-2S cluster (orange Fe and yellow S), Chl a (green) and echinenone (orange) in stick representation. The contour levels of the density maps were adjusted to 0.0233 (unsharpened) and 0.0926 (sharpened), respectively. (E) The arrangement of TMHs in the cytb6f–PetP complex.