Figure 1.
![(A) SDS–PAGE separation of purified cytb6f and the complex reconstituted with PetP (cytb6f–PetP) followed by staining with Coomassie Brilliant Blue confirmed the presence of cytochrome f (cytf), cytochrome b6 (cytb6), the Rieske ISP, subunit IV (subIV) and PetP. (B) Native-PAGE analysis of the same samples showed that the majority of cytb6f was dimeric (labelled as cytb6f [2]) with only a small amount of monomeric complex (labelled as cytb6f [1]). (C) Absorbance spectra of cytb6f (blue) and cytb6f–PetP (red) after reduction with sodium ascorbate (solid line) and sodium dithionite (dotted line). The inset panel shows a magnified view of the contribution of hemes b (564 nm) and f (558 nm).](https://port.silverchair-cdn.com/port/content_public/journal/biochemj/479/13/10.1042_bcj20220124/2/bcj-2022-0124.01.png?Expires=1717060379&Signature=0LqtxLNsSkE2qcC340BSkGNsDKaNeau5L9UK5bbd9BQyLZ-~UpMmccf6L1CYdfWWt~cGXXhr7hrNEBkjCcl1jBYdHUV7lLQ5EmGe~AI2aIaWSQJQnMdxRUvtjGS~Q42IAX~xz0XHnHrPb8AOALLCSPS2fRHamIvIiOFB5bNJhg2ZtyefgM~~iVqOpPg5q79ax0SM1Rz45qTVJYxGgCkIvRvQVOFDFpaIIjEArM15yqpSeqPzVtqRAO3Zc1Har7qZ3OpN~a0ko1pLkU3TcBXLBA-9SWmuJAkKFin6L87EUZMB~Unhi0MGHYzQSuBBK9D~xigBKeQrjb3fWXZADGUg8A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Purification of the Synechocystis cytb6f complex with and without PetP.
(A) SDS–PAGE separation of purified cytb6f and the complex reconstituted with PetP (cytb6f–PetP) followed by staining with Coomassie Brilliant Blue confirmed the presence of cytochrome f (cytf), cytochrome b6 (cytb6), the Rieske ISP, subunit IV (subIV) and PetP. (B) Native-PAGE analysis of the same samples showed that the majority of cytb6f was dimeric (labelled as cytb6f [2]) with only a small amount of monomeric complex (labelled as cytb6f [1]). (C) Absorbance spectra of cytb6f (blue) and cytb6f–PetP (red) after reduction with sodium ascorbate (solid line) and sodium dithionite (dotted line). The inset panel shows a magnified view of the contribution of hemes b (564 nm) and f (558 nm).