Transfection of RAW264.7 cells with LPCAT2 siRNA causes ≥70% decrease in LPCAT2 gene expression and ≥50% decrease in LPCAT2 protein expression (A) *P≤0.05. Fold change shows the optical density of LPCAT2 normalised to GAPDH. Data represent mean of at least three independent experiments (n≥3) ± standard error (A) of Western blots (B) Transfection of siRNA into RAW264.7 cells does not cause a significant difference in total RNA and total protein (C), and in the expression of housekeeping genes ATP5B and GAPDH (D) when compared with cells with no transfected siRNA. Data represent the median of at least three independent experiments (n≥3) ± interquartile range.