SIM2 Chromatin immunoprecipitation assays from cells with dox-induced expression of SIM2s-HF or E19K variant. Enrichment of the 6xCME response element was assessed by qPCR. Graph represents mean of n = 3 independent experiments presented as percent enrichment compared with input. Error bars represent SD. Statistical significance determined by one-way ANOVA. * P ≤ 0.05 *** P ≤ 0.001.