WT or Pkn2Het mice were treated with vehicle or AngII (0.8 mg/kg/d) for 7 days. (a–c), Sections of WT and Pkn2Het mouse hearts were stained with haematoxylin and eosin (H&E), picrosirius red (PSR), or Masson's Trichrome. Representative images are shown with quantification provided below. Individual data points are provided with means ± SEM. Analysis used two-way ANOVA with Holm–Sidak's post-test. (d), RNASeq analysis of hearts from WT and Pkn2Het mice. (i) Summary of differentially-expressed genes (P < 0.01 resulting from AngII treatment. (ii)–(viii) Clusters of DEGs according to function. Results are the mean normalised count values with 95% CI for the n values indicated. Analysis used one-way ANOVA with Holm–Sidak's post-test. (e) Comparison of Tagln (SM22α) mRNA expression assessed by RNASeq and qPCR. Individual data points are shown with means ± SEM. Analysis used two-way ANOVA with Holm–Sidak's post-test.