Mutations disrupt the cell colocalization of FCHSD2 and PDZD7.
(A) GFP-FCHSD2 and PDZD7-mCherry colocalize in the cytoplasm of COS-7 cells in a punctuate pattern. (B–E) T738A/V740R (B) or △PBM (C) disrupts GFP-FCHSD2's colocalization with PDZD7-mCherry; H920A/R928A (D) or △PDZ3 (E) disrupts PDZD7-mCherry's colocalization with GFP-FCHSD2. Scale bar, 20 μm. △PBM or △PDZ3, the truncation of PBM or PDZ3; PBM, PDZ-binding motif.