(A,B) Western blots showing that (A) GFP-FCHSD2 is co-IPed with Myc-PDZD7, and (B) GFP-PDZD7 PDZ3 is co-IPed with Myc-FCHSD2. Expression vectors were transfected into HEK293T cells to express epitope-tagged proteins, and cell lysates were subjected to immunoprecipitation. IP indicates antibody used for immunoprecipitation, and WB indicates antibody used for detection. (C) Expression vectors were transfected into COS-7 cells and the subcellular localization of target proteins was determined using confocal microscopy. Scale bar, 20 μm.