MKN45 cells were treated with or without the indicated concentrations of JB for 24 h. (A) The mitochondrial membrane potential disruption by JB in MKN45 cells measured by flow cytometry (JC-1 staining). (B) Expression levels of apoptosis-related proteins including BAX, BCL-2, Cytochrome c (mitochondrial), Cleaved Caspase-9, Cleaved Caspase-3 were detected by western blotting. Data are expressed as means ± SD of three independent experiments. *P<0.05, **P<0.01 compared with the control group.