MKN45 cells were treated with or without the indicated concentrations of JB for 24 h. (A) Cells stained with AO/EB and viewed under fluorescence microscopy showing apoptosis, Scale bar: 20 µm. (B) The ultrastructure of MKN45 cells were observed by transmission electron microscopy (×13K). (C) Apoptotic rates were quantified using an Annexin V-FITC/PI double staining cytometry. Data are expressed as means ± SD of three independent experiments. *P<0.05, **P<0.01 compared with the control group.