(A) Chemical structure of JB. (B) MKN45 cells were treated with the indicated concentrations of JB for 24 and 48 h, followed by cell viability assessment using MTT assay. (C) MKN45 cells were treated with JB 10, 20, 40 μM for 24 h, and clonal formation rate was detected by colony survival assay. (D) MKN45 cells were treated with JB 10, 20, 40 μM for 24 h, and DNA synthesis was analyzed by EdU incorporation experiment. All data are expressed as means ± SD of three independent experiments. *P<0.05, **P<0.01 compared with the control group.