(A) Coomassie blue stained gel of 100 ng of purified UCKL-1 (lane 1) and UCK2 (lane 2). Single band detected for each: UCKL-1 at 80 kDa and UCK2 at 48 kDa. (B) Western blot analysis with His antibody confirmed purity and isolation of His-tagged UCKL-1 (lane 1) and UCK2 (lane 2). (C) Analysis with UCKL-1 (B-11) antibody (lane 1) showed no cross-reactivity with UCK2 (lane 2). (D) Analysis with UCK2 antibody (lane 2) showed no cross-reactivity with UCKL-1 (lane 1). Blots represent 1 of 3 experiments with identical results.