(A) PBM sequences of TBEV NS5. Full length NS5 comprises residues 2512–3414 based on the TBEV polyprotein (Uniprot accession number Q01299). INT (underlined) was previously reported to be the internal binder [10], and a sequence covering INT with a C-terminal flanking sequence was selected for further investigation. Our findings support the C-terminal binding, not the internal. (B) Schematic outline of TBEV NS5 and Scribble interaction. Red highlights the internal PBM that did not appear to bind. (C) Alphafold structure of full length TBEV NS5 (Apache License, Version 2.0). Methyltransferase in cyan, RNA-dependent RNA polymerase in purple, suggested internal in PBM red and C-terminal PBM in yellow.