(A) Top ten KEGG pathways annotation. KEGG pathway enrichment. The X-axis represents the adjusted P-value, Y-axis is the KEGG pathway. (B) Heatmap of Cd-induced nephrotoxicity and the expression of related genes involved in chemical carcinogenesis pathways. Green: relatively low expression; red: relatively high expression; scale, normalized expression value. (C) PPI diagram of differentially expressed genes related to Cd-induced renal damage and chemical carcinogenesis pathway identified by microarray in the present study. (D) PPI diagram of genes related to the Cd and chemical carcinogenesis pathway in CTD. (E) Venn diagram showing the overlap of differentially expressed genes related to Cd-induced renal damage and chemical carcinogenesis pathway identified by microarray in the present study and the genes related to Cd-related chemical carcinogenesis pathway in the CTD database. (F) Merging of the PPI networks. Yellow nodes indicate the two overlapping genes. (G) CYP1B1 and UGT2B mRNA expression by quantitative reverse transcription polymerase chain reaction (RT-qPCR). (H) CYP1B1 and UGT2B expression by Western blot assay. PPI diagram, the size of a node is determined by the degree of freedom: the greater the degree of freedom, the larger the node. The color of the node is set according to the degree of freedom, going from blue (low degree of freedom) to yellow (high degree of freedom). The thickness of the edge represents the betweenness: the larger the betweenness, the thicker the edge.