Cyc1 level is partially recovered in the suppressors indicating restoration of heme synthesis
Total mitochondrial protein (200 μg) from Δgrx3/4, BY4742 (WT), and suppressors were separated by a gradient SDS/PAGE gel (8–16%). After transfer to nitrocellulose membrane, proteins were immunoblotted with the anti-cytochrome c antibody. Amido black staining of the nitrocellulose membrane was performed to demonstrate success of protein transfer and equal loading of each lane. The CytC content relative to the wild-type level for each strain in duplicate experiments is given above the blot.