Quinone pigment selection for serum 20 lipoprotein subclasses analysis
(A) Representative patterns for resolution of the serum four major lipoprotein classes (black line) into 20 detailed subclasses of lipoproteins (G1-G20, colored lines) using the Gaussian curve fitting technique, following the analysis for TC (A, top) and TG (A, bottom) levels by GP-HPLC system. (B) Correspondence of the detailed subclasses (G1-G20) to the major lipoproteins (CM, VLDL, LDL, and HDL). Black lines are drawn at the boundary time between each subclass. (C) Six Trinder reagent-derived quinone pigments were injected into the GP-HPLC system. Each sample was eluted with running buffer only (without TC and TG reagents) and monitored at 550 nm. The overlaid chromatogram of these pigment solutions (dHDAOS, dDAOS, dADPS, dALPS, dTOOS, and dTOPS) is representative of three separate experiments. A typical free glycerol peak pattern detected using TG reagent is also overlaid to compare its retention time (36 min) with the others.