(A) Five nmole UvrA-mNeonGreen binds extensively to DNA suspended between optically trapped beads. (B) In the presence of 50 µM ATBC, a strong reduction in UvrA-mNeonGreen binding is seen. Counting of the number of bound UvrA-mNeonGreen molecules enabled binding to be quantified. (C) The graph shows the average number of binders recorded in 200 frames (46.5 s). n_{UvrA alone} = 13 (three strands). n_ATBC = 7 (five strands). *P = 0.0002 (unpaired t-test). This shows an approximately sevenfold decrease in binding in the presence of ATBC. All experiments were performed in ABC buffer supplemented with 1 mM ATP and 1 mM DTT.