Portland Press

Figure 2.

$(A–C) are Coomassie-stained SDS–PAGE gels containing protein separated from different stages of the purification of (A) P. putida XylB, (B) BaBAD and (C) AcBAD. The lanes are, +: ladder (PageRuler™ Prestained Protein Ladder, 10 to 180 kDa, ThermoFisher); L: lysate; CL: centrifuged lysate; FT: nickel column flow through; W: nickel column wash; 2–6: nickel column elution fractions. Sizes of ladder markers are given as molecular mass in kilodaltons. (D) shows activity of XylB and BaBAD with either NADH (blue) or NADPH (orange) as the electron donor in the reduction in furfural to furfuryl alcohol, measured by a change in A340 after 1 h.$

Purification of the candidate furfural dehydrogenases.

(A–C) are Coomassie-stained SDS–PAGE gels containing protein separated from different stages of the purification of (A) P. putida XylB, (B) BaBAD and (C) AcBAD. The lanes are, +: ladder (PageRuler™ Prestained Protein Ladder, 10 to 180 kDa, ThermoFisher); L: lysate; CL: centrifuged lysate; FT: nickel column flow through; W: nickel column wash; 2–6: nickel column elution fractions. Sizes of ladder markers are given as molecular mass in kilodaltons. (D) shows activity of XylB and BaBAD with either NADH (blue) or NADPH (orange) as the electron donor in the reduction in furfural to furfuryl alcohol, measured by a change in A₃₄₀ after 1 h.

This Feature Is Available To Subscribers Only