C-terminal LFDE motif residues are not critical for binding LiRAD51.
(A) LiBRC1 mutants and their competition FP assay IC50 and calculated KD values. SE, standard error of fit. (B) Electrophoretic mobility shift assay (EMSA) competition experiments evaluating the ability of LiBRC1 truncation mutants to inhibit LiRAD51 nucleoprotein filament formation. 5 µM LiRAD51 was pre-incubated with GB1-fused LiBRC1 or its truncation mutants, after which FAM-labelled ssDNA (dT60, 100 nM) was added to the reaction. Products were resolved on a 1×TBE 2% agarose gel.