Portland Press

Figure 5

(A) Plots showing inhibition of FXIIa by wild-type Inf4 (closed circles) and Inf4_G25N mutant (open squares). The IC50 values derived from curve fittings are 3.1 ± 0.5 nM for wild-type Inf4 and 54.1 ± 21.8 nM for Inf4_G25N on FXIIa. Data represent the mean ± SEM for triplicate values. (B) Plots showing inhibition of FXIIa by AaTI_Inf4 C1C3 (open circles), AaTI_Inf4 C1C3+N27G (closed circles) and AaTI_Inf4 C1C3+C5C6+N27G (open squares). The IC50 value derived from curve fittings are 4680.6 ± 3834.6 nM for AaTI_Inf4 C1C3, 58.0 ± 14.8 nM for AaTI_Inf4 C1C3+N27G, and 11.4 ± 3.1 nM for AaTI_Inf4 C1C3+C5C6+N27G on FXIIa. Data represent the mean ± SEM for triplicate values.

Inhibitory activities of Inf4 and AaTI_Inf4 C1C3 chimera with mutation at P14′ on FXIIa

(A) Plots showing inhibition of FXIIa by wild-type Inf4 (closed circles) and Inf4_G25N mutant (open squares). The IC₅₀ values derived from curve fittings are 3.1 ± 0.5 nM for wild-type Inf4 and 54.1 ± 21.8 nM for Inf4_G25N on FXIIa. Data represent the mean ± SEM for triplicate values. (B) Plots showing inhibition of FXIIa by AaTI_Inf4 C1C3 (open circles), AaTI_Inf4 C1C3+N27G (closed circles) and AaTI_Inf4 C1C3+C5C6+N27G (open squares). The IC₅₀ value derived from curve fittings are 4680.6 ± 3834.6 nM for AaTI_Inf4 C1C3, 58.0 ± 14.8 nM for AaTI_Inf4 C1C3+N27G, and 11.4 ± 3.1 nM for AaTI_Inf4 C1C3+C5C6+N27G on FXIIa. Data represent the mean ± SEM for triplicate values.

This Feature Is Available To Subscribers Only