Figure 1.
![(A) In Drosophila male GSCs, old (H3–H4)2 are preferentially recycled to the leading strand with yet-to-be identified mechanisms [12]. By default, newly synthesized (H3–H4)2 are incorporated by the lagging strand, thereby generating a biased histone incorporation in a strand biased manner. (B) In mESCs, the role of Mcm2 is shown to recycle old (H3–H4)2 towards the lagging strand [66]. (C) In yeast, old histones recycle towards leading strand via interactions with Dpb3/Dpb4-Polε [68], while the MCM2-ctf4-Polα axis is shown to recycle old (H3–H4)2 towards the lagging strand [67].](https://port.silverchair-cdn.com/port/content_public/journal/biochemsoctrans/50/2/10.1042_bst20200267/1/bst-2020-0267c.01.png?Expires=1717064198&Signature=PLYUif8FZzH3K6rw1WeE31EX0kOsuROUS0pwz7QxtUea3pdE1rVYlSoXNe4MbJmokkBId-EET1xB5SdeWsK9On1ZowNxLYHV9L3N1P3e4jekKkScdXytuikbnF-pewDowvOb1N39J3Hcrc51Qrv~8OVvxfzuGwi95nOkHElS0N7YfVoM4cKhO3-QqI~r~VVZpR9xI4fRfXu3pAjsiwdHLnI~GBtlaFX-BrFdBuCRL0qDNVyRRdmhM16zZWBPvk-nOIpyb2u6xpx1rtSYWObykD5MxLjNqifJ~gPy1DpRknX~JQlVN~Yit6Q-GmRFAyC3xTjPVLPsF9VS86OrIK~ThA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Models for replication-coupled nucleosome assembly (RCNA) highlighting old histone H3–H4 recycling.
(A) In Drosophila male GSCs, old (H3–H4)2 are preferentially recycled to the leading strand with yet-to-be identified mechanisms [12]. By default, newly synthesized (H3–H4)2 are incorporated by the lagging strand, thereby generating a biased histone incorporation in a strand biased manner. (B) In mESCs, the role of Mcm2 is shown to recycle old (H3–H4)2 towards the lagging strand [66]. (C) In yeast, old histones recycle towards leading strand via interactions with Dpb3/Dpb4-Polε [68], while the MCM2-ctf4-Polα axis is shown to recycle old (H3–H4)2 towards the lagging strand [67].