The expression levels of P65, pP65 and IkBα were observed in SW982 under TNF-α-induced conditions; 10 ng/ml induction for 10 min in SW982 cells pretreated with or without 1 µg/ml nicotine. (A) The Western blot of P65, (B) pP65 and (C) IkBα. The band densities indicate significant difference between control, with or without TNF-α and nicotine treatments. The bar graphs represent P65, pP65 and IkBα band densities relative to the total form of each GAPDH and tubulin band intensity determined by densitometric analysis. Values are presented as the mean ± SEM. The significance levels indicate * = P<0.05; versus control 0 min (C) with TNF-α induction for 10 min (CT) and ** = P<0.01 with 24-h pretreatment with nicotine measured using one-way ANOVA. Abbreviations: C, control cells without TNF and nicotine, CT, cells with TNF-α induction for 10 min; nicotine, cells with 24-h pretreated nicotine with TNF-α induction for 10 min.