Green staining indicates CD3 expressing T cells and blue staining denotes the nuclear counterstain, DAPI. Representative photographs of immunofluorescent staining across all treatments. CD3+ T cells were manually counted, and raw values plotted. Scale bar represents 50 μM. Data expressed as mean ± SEM (n=5 mice per group) and analysed by two-way ANOVA with Tukey’s multiple comparisons; * indicates statistical significance P<0.05.