CS exposure causes the depletion of eNOS in the thoracic aorta irrespective of IAV infection
Green staining indicates eNOS-specific expression and blue staining is the nucleus-specific stain DAPI (4′,6-diamidino-2-phenylindole, dilactate). eNOS expression is normalised to the negative control and expressed as percentage change relative to the sham vehicle-treated group. Scale bar represents 50 μM. Data expressed as mean ± SEM (n=5 mice per group) and analysed by two-way ANOVA with Tukey’s multiple comparisons; * indicates statistical significance P<0.05.