Microwave radiation increased AMPKα2 expression and AMPKα (Thr172) phosphorylation in rat and PC12 cell models
(A) Representative protein bands of AMPKα2, p-AMPKα (Thr172), AMPKα and GAPDH in rat hippocampi on 7 days, 14 days and 1 month postradiation. (B,C) The statistical analysis results of the ratio of AMPKα2 to GAPDH and p-AMPKα (Thr172) to AMPKα shown in (A), respectively. (D) Representative protein bands of AMPKα2, p-AMPKα (Thr172), AMPKα and GAPDH in neuron-like cells at 6, 12 and 24 h postradiation. (E,F) The statistical analysis results of the ratio of AMPKα2 to GAPDH and p-AMPKα (Thr172) to AMPKα shown in (D), respectively. (G) The base sequences of pmirGLO-rPrkaa2 3′UTR WT, miR-30a, and pmirGLO-rPrkaa2 3′UTR MUT. (H) The ratio of firefly to Renilla luciferase activity in HEK-293T cells transiently transfected with pmirGLO-rPrkaa2 3′UTR WT, pmirGLO-rPrkaa2 3′UTR MUT and empty pmirGLO vectors in combination with miR-30a mimics or corresponding negative control miRNAs. The data are presented as mean ± SEM. One-way ANOVA with repeated measures was used to analyze the time course, and Student’s t test was used to compare the difference between the two groups SH and MW (B,C,E,F). One-way ANOVA followed by Bonferroni’s post-hoc tests was used to compare multiple groups in (H). *, P<0.05; **, P<0.01; ***, P<0.001 vs the control.