(A) Immunostaining patterns in different glioma grades. (B) IRS among different grades of glioma. (C) mRNA expression of PTMA in representative glioma tissues (n=5, each grade, respectively). (D) Receiver operating characteristic curve for GBM diagnosis using PTMA IRS. (E) Total PTMA protein levels in representative glioma tissues from different grades. *P<0.05, **P<0.01, ****P<0.0001.