Spatial resolution of single-cell transcriptomics, metabolomics and proteomics in germinating barley seeds.
A spatial barcoded map of the barley embryo is obtained using spatial transcriptomics to capture the mRNA transcripts across the embryo tissue section. In parallel, the embryo tissue undergoes tissue dissociation for single-cell RNA sequencing (scRNA-seq), followed by gene expression measurements, dimensionality reduction and clustering of various cell types that constitute the scutellum, plumule and radicle. The single cells are also surveyed for their metabolite and protein contents. Finally, the various single-cell ‘omics measurements are integrated with spatial information to annotate the cell subpopulations [20]. Image adapted from [76] with permission from John Wiley and Sons.