A metagene profile representing the number of RPF reads in a 300-nt region flanking the start of the CDS (CoDing Sequence). In this graph, there are multiple bars increasing vertically in groups of three. Each bar denotes one of the three frames an RPF can be decoded from. There is a clear triplet-like pattern observed in which the read counts follow a pattern of high–low–low, high–low–low, showing a large number of RPFs present in the first frame over subsequent frames, indicating strong RNAse digestion