(A) Schematics of chimeric constructs mG2-Ub and CISD1-Ub. (B and C) Isothermal titration calorimetry of parkin (20 µM) titrated with mG2-Ub (100 µM), CISD1-Ub (212 µM), mG2-pUb (84 µM) or CISD1-pUb (106 µM). The Kd values shown are an average of duplicate titrations. mG2-pUb titrated into parkin had a calculated ΔH = 41 ± 5.5 kJ/mol and ΔS = 260 ± 19 J/mol K. CISD1-pUb titrated into parkin had a calculated ΔH = 30 ± 6.3 kJ/mol and ΔS = 220 ± 22 J/mol K.