Detection of the angiogenic factor VEGF in the retina
Immunohistochemical staining for VEGF (indicated by brown staining) in the retinas of diabetic Akimba mice treated with Vehicle (A–C) or EMPA (D–F). Representative images from 3 mice/group. Magnification 200×; scale bar = 100 μm. Quantitation of VEGF in retina (G); n=4 mice/group.