Figure 3.
![(a) 1H,15N TROSY HSQC spectra of [U-2H,15N]-labeled GII.4 Saga P-dimers in the absence and presence of B-trisaccharide. The insert is highlighting CSPs due to binding of B-trisaccharide to P-dimers. (b) HBGA binding pocket of GII.4 Saga P-dimers (pdb 4 × 06) highlighting the close proximity between the l-fucose residue and R345 causing the strong CSP shown in (a). Figure reproduced [44] under creative commons attributions license.](https://port.silverchair-cdn.com/port/content_public/journal/biochemsoctrans/50/1/10.1042_bst20210526/3/bst-2021-0526c.03.png?Expires=1717072185&Signature=UqxzQna9OdRLVoxmEx6GapCzVcC8shj~8g0Gmvz7hWSbBuvBllXUrfbhxKMwJHSsBQwnOG9alXtPKXx0~Id4j8EBs7gC3AWzkYNG4~mOwbU7oYHJdKi6Xk9iYcOXed~AP~RoG3T9PKUAcfAtg~BWkpuhnZtsQ5StfK6ler3fWUUqiQSHT48NguPlYvbxh6Ncd0q6m-lWj57eDcRXSesuhf-Kf4qMOBT8ntS1QKURJGrytq0LN2-eLxXKBAM~Vc42DxfCF18OHD~FfrSnCcO4kMxCnTnR5izwFnQ7NCsANqQ6Ye6-6HzSF3LfSGLcezbPx2W26aAbw6JlJzLym~biaw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Example for a TROSY HSQC ‘fingerprint’.
(a) 1H,15N TROSY HSQC spectra of [U-2H,15N]-labeled GII.4 Saga P-dimers in the absence and presence of B-trisaccharide. The insert is highlighting CSPs due to binding of B-trisaccharide to P-dimers. (b) HBGA binding pocket of GII.4 Saga P-dimers (pdb 4 × 06) highlighting the close proximity between the l-fucose residue and R345 causing the strong CSP shown in (a). Figure reproduced [44] under creative commons attributions license.