The PI3K/AKT/mTOR pathway was suppressed and the JNK signaling pathway of 4T1 and MDA-MB-231 cells was activated by lobaplatin, microwave hyperthermia and combination therapy
(A) 4T1 and MDA-MB-231 cells were treated with 0, 10 and 20 µg/ml lobaplatin. (B) 4T1 and MDA-MB-231 cells were treated with microwave hyperthermia at 37, 39, 41 and 43°C for 1 h. (C) 4T1 and MDA-MB-231 cells were treated in either the absence or presence of lobaplatin (10 μg/ml) or at an indicated temperature. Twenty-four hours later, P-mTOR, mTOR, P-AMPK, AMPK, P-P70S6K, P-AKT, AKT, P-ERK, P-P38, P-JNK and JNK were detected by Western blot analysis. β-actin was used to confirm that the proteins equal in each lane. Means and standard deviations in the bar charts were from three independent experiments.