(A) Representative images of immunohistochemistry targeting nitrotyrosine (scale bars 20 µm) and (B) its quantification within the glomeruli as well as (C) urinary isoprostane relative to urinary creatinine in the long-term study. Renal cortical gene expression of (D) Nox2 and (E) Nox4 in the short-term study. C, control; D, diabetes; C+MCC950, MCC950-treated control and D+MCC950, MCC950-treated diabetic mice. Asterisks represent P-values for comparisons of the indicated groups: *<0.05, **<0.01 and *** <0.001. n=8–11/group.