Figure 3
(A) Hypoxic HUVECs transfected with miR-24-3p or miR-NC were subjected to migration assays (with or without IL-1β). The migrated cells were counted, and the relative fold change is displayed in a histogram on the right. (B) Hypoxic HUVECs transfected with miR-24-3p or miR-NC were subjected to an invasion assay (with or without IL-1β). The number of invading cells was counted, and the relative fold change is shown in a histogram on the right. (C) Hypoxic HUVECs transfected with si-NC, si-NKAP+miR-NC or si-NKAP+miR-24-3p were subjected to migration assays. The migrated cells were counted, and the relative fold change is displayed in a histogram on the right. (D) Hypoxic HUVECs transfected with si-NC, si-NKAP+miR-NC or si-NKAP+miR-24-3p were subjected to an invasion assay. The number of invading cells was counted, and the relative fold change is shown in a histogram on the right. (E) Hypoxic HUVECs transfected with si-NC, si-NKAP+miR-NC or si-NKAP+miR-24-3p were subjected to a CCK-8 assay. All of the experiments were repeated at least three times. Data represent the mean ± SD; *P<0.05, **P<0.01, ***P<0.001 compared with the control.
IL-1β significantly promotes hypoxic HUVECs migration and invasion

(A) Hypoxic HUVECs transfected with miR-24-3p or miR-NC were subjected to migration assays (with or without IL-1β). The migrated cells were counted, and the relative fold change is displayed in a histogram on the right. (B) Hypoxic HUVECs transfected with miR-24-3p or miR-NC were subjected to an invasion assay (with or without IL-1β). The number of invading cells was counted, and the relative fold change is shown in a histogram on the right. (C) Hypoxic HUVECs transfected with si-NC, si-NKAP+miR-NC or si-NKAP+miR-24-3p were subjected to migration assays. The migrated cells were counted, and the relative fold change is displayed in a histogram on the right. (D) Hypoxic HUVECs transfected with si-NC, si-NKAP+miR-NC or si-NKAP+miR-24-3p were subjected to an invasion assay. The number of invading cells was counted, and the relative fold change is shown in a histogram on the right. (E) Hypoxic HUVECs transfected with si-NC, si-NKAP+miR-NC or si-NKAP+miR-24-3p were subjected to a CCK-8 assay. All of the experiments were repeated at least three times. Data represent the mean ± SD; *P<0.05, **P<0.01, ***P<0.001 compared with the control.

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