(A–D) Western blot analysis of Sirt3, Idh2, and FOXO3a levels in BMSCs. Acetylated Idh2 and FOXO3a were isolated by immunoprecipitation with anti-Idh2 and anti-FOXO3a antibody followed by Western blotting with anti-acetyl-lysine antibody (n=3). (E) Detection of Sirt3 relative activity in BMSCs (n=3), significant differences (P<0.05) were observed among a–c. (F,G) Detection of Idh2 and FOXO3a acetylation level in BMSCs, significant differences (P<0.05) were observed among a–c. All the experiments were repeated at least thrice (n≥3). Data were presented as means ± SD. P<0.05 by one-way ANOVA with Tukey’s post hoc test (three or more groups).